PTC Media
WESTVACO WV5 MEDIUM INCL. VITAMINS
מק"ט:
- A significant improvement in the initiation of embryogenic cultures of loblolly pine from immature seeds was achieved on Westvaco’s WV5 medium defined by Coke with the addition of 30 g/l sucrose, 3 mg/l 2,4-D, 0.5 mg/l BA, 500 mg/l casein hydrolysate, and 1.25 mg/l Gelrite™.
- Up to a threefold increase in embryogenic culture initiation was seen with WV5 medium over other published media.
- WV5 medium was also found suitable for embryo development.
- Shoot cultures of loblolly pine have also been established and micropropagated using Westvaco’s WV5 medium.
- Seedling shoots cultured on WV5 medium with 20 g/l sucrose, 5 g/l activated charcoal, and 8 g/l agar showed improved survival and shoot growth compared to that seen with other published media.
- Shoot quality was excellent and rooting response was good.
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