PTC Media
RUGINI OLIVE MEDIUM
מק"ט:
- The Olive (Olea europaea sativa L.) plays an important role in the economies of countries in the Mediterranean area.
- The in vitro culture of Olive, a particular difficult species to propagate in vitro, required the development of a specific medium formulation.
- Rugini medium is dedicated to the proliferation of the olive shoots.
- The medium has an enriched composition compared to MS. Olive tissues are characterized by a high content of Ca, Mg, S, Cu and Zn.
- The best nitrogen source is a combination of NO3- and NH4+ supplemented with glutamine 2,19 mg/l.
- The better carbon source is mannitol (30-36 gr/l) compared to sucrose.
- A better cytokinin to be used is zeatin: 1 mg/l if filter sterilized, 3-4 mg/l when autoclaved. TDZ and 2iP are less effective.
- Shoots grow more rapidly compared to other media.
- The proliferation rate increases and more tender, sturdier shoots with less basal callus are obtained.
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