PTC Media
LINSMAIER & SKOOG MEDIUM
מק"ט:
- Linsmaier and Skoog have made a systematic study of the organic -requirements of Tobacco cultures in addition to the studies of mineral requirements done by Murashige and Skoog.
- It was found that of all MS vitamins only Thiamine and Inositol are essential.
- The optimum -concen-tration for Thiamine HCl was 0.4 mg/l (MS 0.1 mg/l).
- At a lower concentration growth decreased and the cells became necrotic after 4 weeks.
- Inositol also had a very stimulatory effect on the cell growth but was not as essential as Thiamine.
- All other Murashige & Skoog vitamins were not required for cell growth and could be ommitted without any disadventageous effect.
- Folic acid, p-Aminobenzoic acid, l-Glutamic acid and Ascorbic acid also had a positive influence on cell growth of Nicotiana tabaccum, however the effect was much less than that of Thiamine and Inositol
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