PTC Media
GRESSHOFF & DOY MEDIUM
מק"ט:
- The medium defined by Gresshoff and Doy is developed for growth of haploid callus and plants of Arabidopsis thaliana cultured from the diploid anthers.
- The anthers were removed during the late prophase of meiosis, selecting a genotype favouring callus formation from dividing sporocytes on a high auxin – low kinetin concentration in a fully defined medium.
- Further differentiati-on was induced by transfer to a low auxin – high kinetin medium with a light-dark cycle.
- Haploid callus cultures of tomato, barley and Vitis vinifera have been cultured as well using this method.
כמות:
מקט"ים נוספים:
