Reagents & Biochemicals
X-GLCA CYCLOHEXYLAMMONIUM SALT
מק"ט:
- CAS number 114162-64-0
- Molecular weight C14H13BrClNO7.C6H13N=521.8
- Assay > 98 %
- X-GlcA, 5-Bromo-4-chloro-3-indolyl-ß-D-glucuronic acid is a substrate for ß-D-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as
- a qualitative histochemical marker of specific GUS expressions in cells and tissue. X-GlcA is cleaved by GUS at the ß1 glucuronic bond between
- glucuronic acid and the 5-Bromo-4-chloro-3-indolyl part of X-GlcA via hydrolysis. The enzymatic cleavage of X-GlcA results in the precipitation of a
- water insoluble blue dichloro-di-bromo-indigo precipitate. Color formation requires three separate reactions. After enzymatic turnover, the released in-
- doxyl derivative dimerises and is sub-sequently oxidized to the final indigo dye.
- Storage condition
- Store dry at 2-8 °C
- Soluble in DMSO and DMF
- Protect from light and moisture
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