SCM MEDIUM

מק"ט: S5127.1000

  • 1 kg
  • Bacterial canker is the most important bacterial disease of tomato.
  • The causal organism is Clavibacter michiganensis subsp. michiganensis (Cmm) and this bacterium can be intro-duced by contaminated seeds.
  • For the detection of Cmm, toma-to seeds are first soaked in buffer.
  • Then a stomacher is used for the release of bacteria from the seeds.
  • After the concentra-tion of the bacteria, dilution plating on two semi-selective media is performed.
  • SCM medium is such a semi-selective media.
  • Actually, there are several modifications in use con-cerning the used carbon source, LiCl and the addition of antibi-otics.
  • This medium is used in combination with D2ANX medi-um (D5128).
  • After dilution plating suspected isolates are trans-ferred to YDC.
  • Finally the identity of suspected isolates is determined by a pathogenicity test or PCR. The colonies of Clavibacter michiganensis subsp. michiganensis on SCM are small, light to dark grey, glistening, fluidal and often irregularly shaped.
  • For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic.
  • Brochure can be found at the “Brochure section” at the home page of this website.
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