Phytopathologica & Microbiology
SCM MEDIUM
מק"ט: S5127.1000
- 1 kg
- Bacterial canker is the most important bacterial disease of tomato.
- The causal organism is Clavibacter michiganensis subsp. michiganensis (Cmm) and this bacterium can be intro-duced by contaminated seeds.
- For the detection of Cmm, toma-to seeds are first soaked in buffer.
- Then a stomacher is used for the release of bacteria from the seeds.
- After the concentra-tion of the bacteria, dilution plating on two semi-selective media is performed.
- SCM medium is such a semi-selective media.
- Actually, there are several modifications in use con-cerning the used carbon source, LiCl and the addition of antibi-otics.
- This medium is used in combination with D2ANX medi-um (D5128).
- After dilution plating suspected isolates are trans-ferred to YDC.
- Finally the identity of suspected isolates is determined by a pathogenicity test or PCR. The colonies of Clavibacter michiganensis subsp. michiganensis on SCM are small, light to dark grey, glistening, fluidal and often irregularly shaped.
- For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic.
- Brochure can be found at the “Brochure section” at the home page of this website.
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