Phytopathologica & Microbiology
PTSA MEDIUM
מק"ט: P5135.100
- 1 kg
- PTSA (Peptone Tyrosine Sodium chloride Agar) is a semi-selectivemedium for the detection of Xanthomonas axonopodis pv. phaseoli in bean seed.
- The medium is not very selective in com-parison with mXCP1, but especially colonies from the var. fus-cans are easily recognized on this medium because of their excessive production of visible brown pigment.
- The non-fuscansisolates of Xap grow well on PTSA medium but their recognition is much more difficult due to the lack of pigment production.
- For relatively clean seed lots, PTSA medium is useful, but for saprophyte-rich samples mXCP1 is much more suitable.
- Xap is detected by dilution plating of bacterial extract from seeds on PTSA.
- Then suspected colonies from PTSA should be transferred to YDC.
- Finally, the identity of suspected isolates isconfirmed by a pathogenicity test or PCR.Colonies of Xap var. fuscans are distinguished by brown pigmentation.
- For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic.
- Brochure can be found at the “Brochure section” at the home page of this website.
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