Phytopathologica & Microbiology
PSM MEDIUM
מק"ט: P5134.1000
- 1 kg
- Pseudomonas syringae pv. porri (Pspo) is the causal organism of bacterial blight of leek.
- This pathogen can be seed-borne and therefore the testing of seeds of leek is common.
- Seeds of leek can be saprophyte-rich and this might disguise the presence of Pspo.
- Detection of this bacterium is performed by dilution plating on highly selective media such as KBBC and PSM (Pseudomonas Syringae Medium).
- Putative Pspo colonies are then transferred to KB. Thereafter the identity of the suspected colonies is determined by immunofluorescence microscopy.
- Finally, the identity is determined by a Pspo-specific PCR or a pathogenicity assay using seedlings of leek.
- On PSM the colonies of Pspo are 2-4 mm in diameter, circular with smooth edge, translucent, creamy-yellow to transparant white.
- Note that the color of Pspo colonies is rather variable since the accumulation of bromothymol blue per colony is strongly dependent on the total number of colonies per plate.
- For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic.
- Brochure can be found at the “Brochure section” at the home page of this website.
כמות:
מקט"ים נוספים:
מוצרים נוספים
