MUG TRIHYDRATE

מק"ט:

  • CAS number 6160-80-1
  • Molecular weight C16H16O9.3H2O =406.4
  • 4-Methylumbelliferyl-ß-D-glucu-roni-de trihydrate (4-MUG) is a fluorescent substrate for ß-D-glucuronidase (GUS) encoded by the gusA gene isolated
  • originally from E. coli. Cleavage of the substrate 4-MUG by a ß-glucuronidase activity leads to the gene-ration of the fluorigenic product 4-MU, which can
  • be visualized or detected by irradiation with UV light.(4-Methylumbelliferyl- ß-D-glucuronide (4-MUG) > glucuro-nic acid + 7-hy-droxy-4-me-thyl-couma-
  • rin (MU)) The fluorescence assay allows quantitation of GUS activity by means of a fluorimeter in protein extracts in conjunction with 4-MUG at a peak
  • excitation of 365 nm (UV) and a peak emission of 455 nm (blue).
  • Storage condition
  • Store dry at 2-8 °C
  • Soluble in DMF and DMSO
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