Phytopathologica & Microbiology
MT MEDIUM
מק"ט: M5133.1000
- 1 kg
- The MT (Milk-Tween) Medium is a semi-selective medium for the detection of Pseudomonas syringae pv. syringae (Pss), Pseudomonas savastanoi pv. phaseolicola (Pspha) and Xanthomonas axonopodis pv. phaseoli (Xap) in bean seed.
- The medium relies on the ability of the micro-organisms to hydrolyze casein.
- Suspected isolates are transferred to YDC (Xap) or KB (Pss and Pspha).
- Finally, the identity of suspected col-onies is determined by PCR or a pathogenicity test.
- The colonies of Pspha and Pss are cream white, flat circular, 4-5 mm in diameter and produce a blue fluorescent pigment under UV light.
- Xap colonies (3 – 3.5 mm in diameter) are yellow, non fluorescent and typical two zones surround colonies: a bigger, clear zone of casein hydrolysis and a smaller zone of Tween 80 lipolysis.
- Xap var. fuscans (1 – 2 mm in diameter) produces a brown pigment within 5 days.
- For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic.
- Brochure can be found at the “Brochure section” at the home page of this website.
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