Phytopathologica & Microbiology
MCS20ABN MEDIUM
מק"ט: C5122.1000
- 1 kg
- CS20ABN has been developed by Chang et al. to isolate Xanthomonas campestris pv. campestris (Xcc) from crucifer seeds.
- The original medium recipe allowed the quick isolation of most isolates of Xcc.
- However, the recovery of some isolates of Xcc was poor due to pH-dependent sensitivity to neomycin.
- In the modified version, the pH is lowered to 6.5 by the addi-tion of extra potassium dihydrogen phosphate.
- This modification improved the recovery of some neomycin-sensitive isolates considerably.
- Contaminated seed lots can be detected by dilution plating of the bacterial extract on mCS20ABN and mFS.
- Suspected isolates are then transferred to YDC.
- Finally, the identity of the suspected isolates can be determined by a pathogenicity test using brassica seedlings.
- The colonies of Xcc and Xanthomonas campestris pv. armoraciae are yellow, mucoid and surrounded by a zone of starch hydrolysis.
- or detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic.
- Brochure can be found at the “Brochure section” at the home page of this website.
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