Phytopathologica & Microbiology
KBZ MEDIUM
מק"ט: K5129.1000
- 1 Kg
- Bacterial speck of tomatoes is caused by the bacterium Pseudomonas syringae pv. tomato (Pst).
- The bacterium can be introduced by the use of Pst-contaminated seeds.
- Therefore, detection of Pst in seeds of tomato is common.
- For the detection of Pst, seeds are first soaked in buffer.
- Then a stomacher is used for the release of bacteria from the seeds.
- The bacteria are concentrated by centrifugation.
- Then dilution plating on two semi-selectice media KBZ and KBBC is performed.
- Suspected colonies are transferred to KB and finally identified by PCR or a pathogenicity assay.
- Pst forms small, flat and pink-colored colonies on KBZ after ca. 5 days.
- For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic.
- Brochure can be found at the “Brochure section” at the home page of this website.
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