תוספים - Supplements
Corning HEPES
(Corning - קורנינג)
מק"ט:
- HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2.
- Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer.
- Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.
- The recommended working concentration is 10-25 mM.
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