מדיה קלאסית - Classical Media
Corning 50L DMEM (Dulbecco’s Modified Eagle’s Medium), Powder
(Corning - קורנינג)
מק"ט: 50-003-PC
- [+] 4.5 g/L glucose, L-glutamine, sodium pyruvate[-] sodium bicarbonate Technical Advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME.
- The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells.
- DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine.
- DMEM 50-003-PB and -PC contain 4.5 g/L glucose, L-glutamine, and sodium pyruvate and are formulated without sodium bicarbonate.
- The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells.
- Since then, it has been modified to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells.
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