Corning 500mL/1L DMEM (Dulbecco’s Modified Eagle’s Medium)

Corning 500mL/1L DMEM (Dulbecco’s Modified Eagle’s Medium)

(Corning - קורנינג)

מק"ט:

  • [+] 4.5 g/L glucose[-] L-glutamine, sodium pyruvate Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation.
  • Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME.
  • The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells.
  • DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 15-017-CV and -CM contain 4.5 g/L glucose and do not contain sodium pyruvate and L-glutamine.
  • The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells.
  • Since then, it has been modified to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells.
  • With 4.5 g/L glucose without L-glutamine and sodium pyruvate.
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