ריאגנטים - Reagents
Corning 100mL Trypan Blue Solution, 0.4% (w/v) in PBS, pH 7.5 ± 0.5
(Corning - קורנינג)
מק"ט: 25-900-CI
- 0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4) Technical Advantage: Can be used for a variety cell counting methods requiring information on cell count and viability.
- Trypan Blue is the most common stain used in cell count and viability assays.
- Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue.
- Cells should be in suspension as single cells in buffered saline before counting.
- Trypan blue can be used in assays providing total cell count, viable cell or nonviable cell count alone.
- This solution is provided at the common concentration of 0.4% (w/v).
כמות:
מקט"ים נוספים:
