Corning 100 mL HAT (Hypoxanthine, Aminopterin, Thymidine), 50x

Corning 100 mL HAT (Hypoxanthine, Aminopterin, Thymidine), 50x

(Corning - קורנינג)

מק"ט: 25-046-CI

  • HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells.
  • The combination of aminopterin, a drug that acts as a powerful folate metabolism inhibitor, with hyproxanthine and thymidine, which are intermediates in DNA synthesis, provides a form of artificial selection for cells containing functioning hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and thymidine kinase (TK).
  • These enzymes are involved in DNA synthesis and cell division.
  • HAT medium is often used in the preparation of monoclonal antibodies.
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